Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase

doi: 10.1186/s13046-018-0805-4

Figure Lengend Snippet: Proposed mechanistic scheme: AKBA suppresses glioblastoma by arresting cell cycle at G2/M phase. AKBA activates p21 which decreases expression of FOXM1 and its downstream target genes including Aurora A, Aurora B, PLK1, and cyclin B1. Inhibiting Aurora A suppresses the expression of PLK1 which leads to the inactivation of CDK1/cyclin B1 complex and arrests the cell cycle at the G2/M checkpoint. Inhibiting Aurora B decreases expression of TOP2A and inhibits mitosis

Article Snippet: After blocking with 5% fat-free milk in TBST for 1 h, membranes were immunoblotted with primary antibodies to caspase3, cleaved-caspase3, PARP, cleaved-PARP, p21, p-RB, PLK1, p-CDK1, and Aurora B (Cell Signaling Technology); p53, FOXM1, Aurora A, CDC-25C, CDK-1, cyclin B1, TOP2A, and GAPDH (Proteintech) at the appropriate dilutions with gentle shaking overnight at 4 °C.

Techniques: Expressing

Journal: Cancers

Article Title: Aurora Kinase A Inhibition Potentiates Platinum and Radiation Cytotoxicity in Non-Small-Cell Lung Cancer Cells and Induces Expression of Alternative Immune Checkpoints.

doi: 10.3390/cancers16162805

Figure Lengend Snippet: Figure 5. Knockdown of AURKA increases apoptosis induced by cisplatin or radiation. (A) Expres- sions of C-PARP, AURKA, and GADPH were shown in PC9 and A549 cells with and without the treatment of cisplatin and/or doxycycline for 48 h. (B) Expressions of C-PARP, AURKA, and GADPH were shown in PC9 and A549 cells (with and without the treatment of radiation and/or doxycycline for 48 h). The ratios of band intensities divided by GAPDH are noted at the top of each protein band.

Article Snippet: For Western blot analysis, antibodies against AURKA (catalog no. 91590), phosphoAurora A (Thr288) (catalog no. 3079), total PARP (catalog no. 9532), cleaved PARP (Asp214) (catalog no. 5625), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 5174) were sourced from Cell Signaling Technology (Boston, MA, USA).

Techniques: Knockdown

Journal: Cancers

Article Title: Aurora Kinase A Inhibition Potentiates Platinum and Radiation Cytotoxicity in Non-Small-Cell Lung Cancer Cells and Induces Expression of Alternative Immune Checkpoints.

doi: 10.3390/cancers16162805

Figure Lengend Snippet: Figure 6. Inhibiting AURKA by MLN8237 induces apoptosis and senescence. (A) Western blotting: the expression of cleaved PARP and GADPH in PC9 and A549 cells with and without treatment of cisplatin and/or MLN8237 for 48 h. (B) Representative picture of staining of senescence-associated beta-galactosidase in PC9 and A549 cells with and without treatment of cisplatin and/or MLN8237. (C) Quantitative analysis of staining of senescence-associated beta-galactosidase in PC9 and A549 cells with and without treatment of cisplatin and/or MLN8237.

Article Snippet: For Western blot analysis, antibodies against AURKA (catalog no. 91590), phosphoAurora A (Thr288) (catalog no. 3079), total PARP (catalog no. 9532), cleaved PARP (Asp214) (catalog no. 5625), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (catalog no. 5174) were sourced from Cell Signaling Technology (Boston, MA, USA).

Techniques: Western Blot, Expressing, Staining